In this episode of Let's Talk Micro, we continue our gram-negative cocci series by focusing on what to do after the Gram stain. You've identified gram-negative cocci—now what?

We take a practical, bench-focused approach to working these organisms up, covering key culture media like blood agar, chocolate agar, and selective media such as Modified Thayer-Martin and Martin-Lewis. We also review collection and transport considerations, including JEMBEC and CO₂ requirements for Neisseria gonorrhoeae.

We then dive into commonly used tests, including ONPG for differentiating Neisseria lactamica and Neisseria meningitidis, the CTA test and its limitations, and the butyrate esterase test for identifying Moraxella catarrhalis.

This episode is all about building confidence at the bench—connecting what you see to what you do next.

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In this episode of Let's Talk Micro, we kick off a new series on gram-negative cocci by starting where it all begins—the Gram stain.

Seeing gram-negative diplococci on a smear can feel intimidating, especially when you're on the bench and need to make the right call. In this episode, we focus on how to approach that moment with confidence. We walk through the key organisms you'll encounter, including Neisseria, Moraxella, and Veillonella, and discuss how morphology, specimen source, and clinical context all come together to guide your thinking. We also revisit a correction from a previous episode regarding carbohydrate utilization in Neisseria species.

This episode is all about building a strong foundation—how to think before jumping to identification.

Stay tuned for the next episode, where we'll dive into the media and tests used to identify these organisms in the lab.

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The CLSI M100 gets updated every year—but what do those changes actually mean for the microbiology lab?

In this episode of Let's Talk Micro, Luis is joined by Dr. April Bobenchik to break down the latest updates in the CLSI M100 2026 (36th edition) and how they translate into real-world lab practice.

They walk through important breakpoint changes, including aztreonam–avibactam and updates for Acinetobacter, along with new additions like trimethoprim-sulfamethoxazole breakpoints for beta-hemolytic streptococci. Another notable update this year is the updated anaerobic antibiogram and how it can be used to help guide decisions in the lab. The conversation also touches on antimicrobial tiers, why some breakpoints are temporarily removed, and practical ways labs can approach implementing new updates.

If you work in clinical microbiology, this episode will help you stay current and confident with the latest M100 changes.

Access CLSI M100 for free: https://em100.edaptivedocs.net/Login.aspx

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Luis here with a quick update! We're taking a short break and will return the week of March 23rd. During this time, it's a great opportunity to explore the archive—Let's Talk Micro has over 200 episodes covering many topics in clinical microbiology. New episodes will be back soon. Thanks for listening and for being part of the Let's Talk Micro community.

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• Website: letstalkmicro.com

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Wound cultures are some of the most complex specimens encountered in the clinical microbiology laboratory. Unlike more straightforward cultures, wound specimens often contain multiple organisms, making it challenging to determine which microbes are clinically significant and which represent colonization.

In this episode of Let's Talk Micro, Luis is joined by Dr. Christopher Doern and Dr. Alexandra Bryson to discuss the wound culture guidelines from the Clinical Microbiology Procedures Handbook (CMPH) and how they can help laboratorians navigate these challenging cultures.

Together, they explore practical approaches to interpreting polymicrobial wound cultures, deciding which organisms to work up, and balancing thorough reporting with antimicrobial stewardship. The discussion also covers specimen collection challenges, the role of Gram stains in evaluating specimen quality, guidance on working up common wound pathogens, and strategies to streamline laboratory workflows.

Whether you're a student, a new microbiology technologist, or an experienced laboratorian, this episode offers practical insights to help you confidently approach wound cultures and apply CMPH guidance at the bench.

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• Website: letstalkmicro.com

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The Gram stain is one of the most trusted tools in clinical microbiology—but it doesn't always tell the full story.

In this Micro Minutes episode, we break down why Gram stains can sometimes be misleading and how factors like culture age, antibiotic exposure, decolorization, morphology, and arrangement can change what you see under the microscope. We also touch on classic troublemakers like Acinetobacter and tiny coccobacilli such as Pasteurella and Haemophilus, and how careful observation can keep you from jumping to the wrong conclusion.

Short, focused, and straight from the bench—this episode is all about learning how to interpret Gram stains beyond just purple and pink.

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In this episode of Let's Talk Micro, we step beyond the microbiology bench to talk laboratory medicine, advocacy, and storytelling with fellow medical laboratory scientists and podcasters Stephanie Whitehead and Aaron Odegard.

We explore how their work on the SuperStat Podcast is helping amplify the voice of the laboratory through conversations on leadership, access, and visibility. The discussion also highlights Little Lab Learners: ABCs of Laboratory Medicine, a children's book created to introduce laboratory medicine early and inspire future lab professionals.

Together, we talk about lab career paths, the impact of automation and the pandemic on lab visibility, and why advocacy doesn't look the same for everyone—but matters now more than ever. This episode is a reminder that the lab's story doesn't end at the bench—it's one we all help tell.

Little Lab Learners: ABCs of Laboratory Medicine is a children's book created to introduce laboratory medicine early and help kids see lab professionals as scientists, problem-solvers, and essential members of healthcare.

Learn more about the book and the Little Lab Learners series here:
👉 https://labcares.org/Little_Lab_Learners/

Listen to the SuperStat Podcast on Spotify:
👉 https://open.spotify.com/show/5wYrCFh375KclnCFY8YyN2?si=b681fc3748df48e4

Stay connected with Let's Talk Micro:

• Website: letstalkmicro.com

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In this episode of Let's Talk Micro, Luis is joined by Dr. Joel Mortensen to discuss a study examining intestinal parasites in pediatric patients in Guinea, West Africa.

They dive into the real-world challenges of diagnosing parasitic infections in resource-limited settings, including reliance on stool microscopy, the use of rapid antigen tests, and how seasonal changes influence infection rates. Dr. Mortensen walks us through the study design, key findings, and what the data revealed about protozoal infections, as well as the role of microscopy in identifying helminths in this setting.

This conversation highlights the critical role of clinical microbiology in global health, the importance of diagnostic context, and how laboratory data can directly inform patient care and public health strategies in underserved regions.

If you enjoyed this conversation, be sure to check out previous episodes featuring Dr. Mortensen, where we dive deeper into his work in Guinea, laboratory capacity building, and global health challenges in resource-limited settings:

• 🎙️ Episode 107: A Pediatric Hospital in Guinea – Pt. 1
  https://directory.libsyn.com/episode/index/id/35868465

• 🎙️ Episode 108: A Pediatric Hospital in Guinea – Pt. 2
  https://directory.libsyn.com/episode/index/id/35868460

These episodes provide important background on the hospital, training efforts, and the broader impact of this work.

Learn more about Sacre Coeur Hospital and the pediatric care and laboratory work being done in Guinea:
👉 https://www.hopeignited.org/sacre-coeur

If you're interested in learning more about this work, collaborating, or supporting efforts in Guinea, you can reach out directly to Dr. Joel Mortensen:

📧 joelmortensen@gmail.com

You can also contact me at letstalkmicro@gmail.com, and I'm happy to help connect you.

Stay connected with Let's Talk Micro:

• Website: letstalkmicro.com

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